Scientific Articles


Francisco, V, Ferreira, L.

Effective conjugation of RNA molecules to nanocarriers for efficient and controlled delivery of RNA-based therapies

(submitted), 2021

Estronca, L, Francisco, V, Pitrez, P, Honorio, I, Carvalho, L, Blersch, J, Rai, A, Nissan, X, Simon, U, Graos, M, Saude, L, Ferreira, L

Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions

(submitted), 2021

Marta Esteves, Ricardo Abreu, Hugo Fernandes, Catarina Serra-Almeida, Ana ClaraCristóvão, Cláudia Saraiva, Raquel Ferreira, Lino Ferreira, Liliana Bernardino

MicroRNA-124-enriched exosomes as a promising therapeutic approach for Parkinson’s disease

(submitted), 2021

Rai, A*, Ferreira, L.

Peptide decorated gold nanoparticles for biomedical applications

(submitted), 2021

Abreu, R, Ramos, C, Becher, C, Jesus, C, Martins, PC, Martins, PAT, Moreno, MJ,Fernandes, H* , Ferreira, L*

Post-isolation enrichment of small extracellular vesicles with microRNAs

(submitted), 2021

Conniot, J, Talebian, S, Simoes, S, Ferreira, L*, Conde, J*

Revisiting gene delivery to the brain: silencing versus editing

(submitted), 2021


Pitrez, PR, Estronca, L, Vazão, H, Harhouri, K, Thornton, D, Navarro, C, Egesipe, A-L,Lévy, N, Magalhães, JP, De Sandre-Giovannoli, A, Nissan, X, Rosell, A, Ferreira L

A chip to study the vulnerability of progeroid smooth muscle cells to flow shear stress

NatureCommunications 11, 4110, 2020

Blersch, J, Francisco, V, Rebelo, C, Jimenez, A, Henriques-Antunes, H, Gonzato, C,Pinto, S, Liedl, K, Haupt, K, Ferreira, L

A light-activatable nanoparticle library for the controlled release of non-coding RNAs

Angew Chem Int Ed 59(5), 1985-1991, 2020

Blersch, J, Francisco, V, Rebelo, C, Jiménez-Balsa, A, Antunes, H, Pinto, S, Simões, S, Rai, A, Ferreira, L

A light-triggerable formulation to control the stability of pro-angiogenic transcription factor hypoxia inducible factor-1 (HIF-1)

Nanoscale 12, 9935-9942, 2020

Ferreira, R, Napoli, J, Enver, T, Bernardino, L*, Ferreira, L*

Advances and challenges in retinoid delivery systems in therapeutic and regenerative medicine

Nature Communications 11, 4265, 2020

Abreu, R, Fernandes, H, Martins, PC, Sahoo, S, Emanueli, C, Ferreira, L

Native and engineered extracellular vesicles for cardiovascular therapeutics

Nature Reviews Cardiology (in press), 2020

Bernardo, S, Donato, H, Ferreira, L, Freitas, JS

Permeability of the blood brain barrier through the phases of ischemic stroke and relation with clinical outcome: protocol for a systematic review

BMJ Open (in press), 2020

Katarzyna Buczak, Joanna M. Kirkpatrick, Felicia Trockenmueller, Deolinda Santinha,Lino Ferreira, Stephanie Roessler, Stephan Singer, Alessandro Ori, and Martin Beck

Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry

Nature Protocols 15, 2956-2979, 2020


Rosa, S, Pitrez, P, Fernandes, H*, Ferreira, L*

A high-throughput screening method to identify compounds displaying human vascular embryonic toxicity

Current protocols in Stem Cell Biology, 50(1), e93, 2019

Abecasis, B, Gomes-Alves, P, Rosa, S, Gouveia, P, Ferreira, L, Serra, M, Alves, PM

A human induced pluripotent stem cell derived cardiac model: new inisghts in the crosstalk between cardiomyocytes and endothelial cells

Biotechnology and Bioengineering (in press), 2019

Francisco, V, Lino, M, Ferreira, L

A modular system for the delivery of small biomolecules based in supramolecular modified gold nanorods

Journal of Nanobiotechnology (in press), 2019

Yao, T-T, Wang, J, Yu, W-J, Gao, Q, Ferreira, L, Ren, K-F, Ji, J

A photodynamic antibacterial spray-coating based on the host-guest immobilization of the photosensitizer methylene blue

Journal of Materials Chemistry B (in press), 2019

Banerjee, A, Alves, V, Rondão, T, Sereno, J, Lino, M, Ribeiro, A, Abrunhosa, A*, Ferreira, L*

A positron-emission tomography (PET)/magnetic resonance imaging (MRI) platform to track in vivo small extracellular vesicles

Nanoscale 11, 13243-13248, 2019

Querido, MM, Felgueiras HP, Rai, A, Monteiro, C, Oliveira, D, Ferreira, L, Martins MCL

Antimicrobial coating to prevent infections associated with polyurethane without inducing platelet adhesion and activation

Advanced Materials Interface (in press), 2019

Ng, KS, Smith, JA, McAteer, MP, Mead, BE, Ware, J, Jackson, FO, Ferreira, L, Bure, K, Rowley, JA, Reeve, B, Brindley, DA, Karp, JM

Bioprocess decision support tool for scalable manufacture of extracellular vesicles

Biotechnology and Bioengineering, 116(2), 307-319, 2019

Praça, C, Rosa, S, Sevin, E, Cecchelli, R, Dehouck, M-P, Ferreira, L

Derivation of brain-like endothelial cells from human pluripotent stem cell-derived endothelial progenitor cells

Stem Cell Reports (in press), 2019

Rosa, S, Praça, C, Pitrez, PR, Gouveia, PJ, Aranguren, XL, Ricotti, L, Ferreira, L

Functional characterization of iPSC-derived arterial- and venous-like endothelial cells

Scientific Reports 9, 3826, 2019

Chen, X-C, Huang, W-P, Ferreira, L, Ren, K-F, Ji, J

Spatially Confining Surface Roughness on Exponentially Growing Polyelectrolyte Multilayer Films

Advanced Materials Interfaces (in press), 2019

Henriques-Antunes, H*, Cardoso, RMS,* Zonari, A, Correia, J, Leal, EC, Jimenez-Balsa, A, Lino, M, Barradas, A, Kostic, I, Gomes, C, Karp, JM, Carvalho, E, Ferreira, L

The kinetics of small extracellular vesicle delivery impacts skin tissue regeneration

ACS Nano (in press), 2019

Ferreira, L.

What human blood-brain barrier models can tell us about BBB function and drug discovery?

Expert Opinion on Drug Discovery (in press), 2019


Praça, C*, Rai, A*, Santos, T, Cristovão, AC, Pinho, SL, Cecchelli, R, Dehouck, M-P, Bernardino, L, Ferreira, L

A nanoformulation for the preferential accumulation in adult neurogenic niches

Journal Controlled Release, 284, 57-72, 2018

Ferreira, AF, Comune, M, Rai, A, Ferreira, L, Simões, PN

Atomistic-level investigation of a LL37 conjugated gold nanoparticle by well-tempered metadynamics

Journal of Physical Chemistry Part: B, 122(35), 8359-8366, 2018

Gomes, AM, Kurochkin, I, Chang, B, Daniel, M, Law, K, Satija, N, Lachmann, A, Wang, Z, Ferreira, L, Ma´ayan, A, Chen, BK, Papatsenko, D, Lemischka, IR, Moore, KA, Pereira, C-F

Coopertative transcription factor induction mediates hemogenic reprogramming

Cell Reports, 25(10), 2821-2835, 2018

João Sargento-Freitas, Sezin Aday, César Nunes, Miguel Cordeiro, Ana Gouveia, Fernando Silva, Cristina Machado, Bruno Rodrigues, Gustavo Cordeiro, Carlos Ferreira, André Amorim, Susana Sousa, Ana Catarina Gomes, Miguel Castelo-Branco, Lino Ferreira* and Luís Cunha*

Endothelial progenitor cells enhance subacute blood-brain barrier permeability in ischemic stroke

Neurology, 90(2), e127-e134, 2018

João Sargento-Freitas, Sezin Aday, César Nunes, Miguel Cordeiro, Ana Gouveia, Fernando Silva, Cristina Machado, Bruno Rodrigues, Gustavo Cordeiro, Carlos Ferreira, Miguel Castelo-Branco, Lino Ferreira* and Luís Cunha*

Endothelial progenitor cells influence acute and subacute stroke hemodynamics

Journal of the Neurological Sciences, 385, 119-125, 2018

Marta Machado-Pereira, Tiago Santos, Lino Ferreira, Liliana Bernardino, Raquel Ferreira

Intravenous administration of retinoic acid-loaded polymeric nanoparticles prevents ischemic injury in the immature brain

Neurosciences Letters, 673, 116-121, 2018

Jimenez-Balsa A, Pinto S, Quartin E, Lino MM, Francisco F, Ferreira L

Light-activatable polymeric nanoparticles for intracellular delivery of proteins and small molecules

Bioconjugate Chemistry, 29 (5), pp 1485–1489, 2018

Lino MM, Ferreira L

Light-triggerable formulations for the intracellular controlled release of biomolecules

Drug Discovery Today, 23(5), 1062-1070, 2018

Cláudia Saraiva, Daniela Talhada, Akhilesh Rai, Raquel Ferreira, Lino Ferreira, Tadeusz Wieloch, Liliana Bernardino*, Karsten Ruscher*

MicroRNA-124-loaded nanoparticles protect and increase differentiation of neural stem cells in vitro but do not contribute to stroke outcome

PLoS One, 13(3): e0193609, 2018

Lino MM, Simões S, Vilaça A, Antunes H, Zonari A, Ferreira L

Modulation of angiogenic activity by light-activatable nanocarriers carrying miRNAs

ACS Nano, 12(6), 5207-5220., 2018

Lima, A.F., May G., Colunga J., Pedreiro, S., Paiva, A., Ferreira, L., Enver T., Iborra F.J. , Pires das Neves, R.

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Scientific Reports, 8, 7210, 2018

Sargento-Freitas, João, Pereira, Anabela, Gomes, André, Freire, Paula, Matos, Teresa, Cardoso, Carla, Silva, Fernando, Cordeiro, Gustavo, Nunes, César, Galego, Orlando, Carda, José, Branco, João, Lourenço, Vítor, Cunha, Luís, Ferreira, Lino

STROKE34 study protocol: A randomized controlled phase IIa trial of intra-arterial CD34+ cells in acute ischemic stroke

Frontiers Neurology, 9, 302, 2018

Pinho S#, Macedo MH#, Rebelo C#, Sarmento B*, Ferreira L*

Stem cells as vehicle and target of nanoparticles

Drug Discovery Today, 23(5), 1071-1078, 2018

Patricia R. Pitrez, Luis Estronca, Helena Vazão, Anna-Laure Egesipe, Claire Navarro, Annachiara De Sandre-Giovannoli, Xavier Nissan, Lino Ferreira*

Substrate topography modulates cell ageing in a Progeria model

ACS Biomaterials Science and Engineering, 4(5), 1498-1504, 2018


Marta Machado-Pereira, Tiago Santos, Lino Ferreira, Liliana Bernardino, Raquel Ferreira

Anti-inflammatory strategy for M2 microglial polarization using retinoic acid-loaded nanoparticles

Mediators of Inflammation, ID 6742427, 2017

Comune M., Rai A., Chereddy K.K., Pinto S., Aday S., Ferreira A.F., Cunha R., Rodrigues R., Simões P.N., Preat V., Ferreira, L.

Antimicrobial peptide-nanoscale therapeutic formulation with high skin regenerative potential

Journal Controlled Release, 262, 58-71, 2017

Ribas, J., Zhang, Y.S., Pitrez, P.R., Leijten, J., Miscuglio, M., Rouwkema, J., Dokmeci, M., Nissan, X., Ferreira, L.*, Khademhosseini, A.*

Biomimetic strain exacerbates aging in a progeria-on-a-chip model

Small, 13, 1603737 (*Authors contributed equally). DOI:10.1002/smll.201603737, 2017

Machado-Pereira, M., Santos, T., Ferreira, L., Bernardino, L., Ferreira, R.

Challenging the great vascular wall: can we envision a simple yet comprehensive therapy for stroke?

J Tissue Engineering and Regenerative Medicine, doi: 10.1002/term.2427, 2017

Teresa M. Ribeiro-Rodrigues, Tiago L. Laundos, Justin C Mason, Paulo Per, Rita Pereira-Carvalho, Ricardo Pereira, Vanessa Coelho-Santos, Ana Paula Silva, Rosa Fernandes, Mónica Zuzarte, Francisco J. Enguita, Marina C. Costa, Perpetua Pinto-do-Ó, Marta Teixeira Pinto, Pedro Gouveia, Lino Ferreira, Justin C Mason, Paulo Pereira, Brenda R Kwak, Diana S Nascimento, Henrique Girão

Exosomes secreted by cardiomyocytes subjected to ischemia promote cardiac angiogenesis

Cardiovascular Research (in press), 2017

Gouveia P, Rosa S, Ricotti L, Abecassis B, Almeida HV, Monteiro L, Nunes J, Sofia Carvalho F, Serra M, Luckin S, Leonidovitch Kholkin A, Marques Alves P, Jorge Oliveira P, Carvalho R, Menciassi A, Neves RP, Ferreira LS

Flexible nanofilms coated with aligned piezoelectric microfibers preserve the long-term contractility of cardiomyocytes

Biomaterials, 139, 213-228, 2017

Vazão H*, Rosa S*, Barata T, Costa R, Pereira P, Honório I, Papatsenko D, Benedito R, Saris D, Khademhosseini A, Pereira CF, Mercader N, Fernandes H, Ferreira L

High-throughput identification of small molecules that affect human embryonic vascular developmen

PNAS, 114(15), E3022-E3031. (*Authors contributed equally), 2017

Lino, M. M., Simões, S., Pinho, S., Ferreira, L.

Intracellular delivery of more than one protein with spatio-temporal control

Nanoscale, 9, 18668-18680, 2017

Tiago Santos, Raquel Ferreira, Emanuel Quartin, Carlos Boto, Cláudia Saraiva, José Bragança, João Peça, Cecília Rodrigues, Lino Ferreira, Liliana Bernardino

Light potentiates retinoic acid-induced neuronal differentiation of neural stem cells

Acta Biomaterialia, 59, 293-302, 2017

Carlos Boto, Emanuel Quartin, Yijun Cai, Alberto Martin-Lorenzo, Maria Begona Garcia Cenador, Sandra Pinto, Rajeev Gupta, Tariq Enver, Isidro Sanchez-Garcia, Deng Li, Ricardo Neves, Lino Ferreira

Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation

Nature Communications, 8:15204, DOI: 10.1038/ncomms15204, 2017

Luís Miguel Monteiro, Lino Ferreira, Perpétua Pinto-do-Ó, Diana S Nascimento

Restoring Heart Function and Electrical Integrity: Closing the Circuit

Regenerative Medicine. doi:10.1038/s41536-017-0015-2, 2017

Sezin Aday, Marie Besnier, Janet Zoldan, Laura Carreto, Jaimy Saif, Rui Fernandes, Tiago Santos, Liliana Bernardino, Robert Langer, Costanza Emanueli, Lino Ferreira

Synthetic microparticles conjugated with VEGF165 improve the survival and function of endothelial progenitor cells via microRNA-17 inhibition

Nature Communications, 8, 747, 2017


Lo Cícero, A., Jaskowiak, A.-L., Egesipe, A.-L-, Tournois, J., Brinon, B., Pitrez, P.R., Ferreira, L., Giovannoli, A. S., Levy, N., Nissan, X.

A high throughput phenotypic screening reveals compounds that counteract premature osteogenic differentiation of HGPS iPS-derived mesenchymal stem cells

Scientific Reports, 6:34798, 2016

Rai, A, Pinto, S., Evangelista, M., Ferreira, L.

Antimicrobial peptide permanently immobilized on surfaces with high activity in the presence of serum and low cytotoxicity against human cells

Acta Biomaterialia, 3, 64-77, 2016

André Ferreira, Akhilesh Rai, Lino Ferreira, Pedro Simões

Atomistic insights into the interaction of a cecropin-mellitin hybrid with a gold surface

European biophysical journal, DOI 10.1007/s00249-016-1160-z, 2016

Ribas, J., Sadeghi, H., Manbachi, A., Leijten, J., Brinegar, K., Zhang, Y.S., Ferreira, L., Khademhosseini, A.

Cardiovascular organ-chip platforms for drug discovery and development

Applied In Vitro Toxicology, 2(2), 82-96, 2016

Claudia Saraiva#, Catarina Almeida#, Raquel Ferreira, Tiago Santos, Lino Ferreira*, Liliana Bernardino*

Drug delivery into the brain: the blood brain barrier challenge and the nanomedicine solutions

J Controlled Release, 235, 34-47. #,* Authors contributed equally, 2016

Hajar Maleki*, Akhilesh Rai*, Sandra Pinto*, Marta Evangelista, António Portugal, Luísa Durães, Mohammad Imani, Abolreza Simchi, Lino Ferreira

High antimicrobial activity and low human cell cytotoxicity of core-shell magnetic nanoparticles functionalized with an antimicrobial peptide

ACS Applied Materials and Interfaces, 8(18), 11366-78. *Authors contributed equally, 2016

Cláudia M. Saraiva, José M. Paiva, Tiago Santos, Lino S. Ferreira, Liliana Bernardino

MicroRNA-124 loaded nanoparticles enhance murine subventricular zone neurogenesis

J Controlled Release, 235, 291-305, 2016

Tiago Santos, Carlos Boto, Cláudia Saraiva, Liliana Bernardino*, Lino Ferreira*

Nanomedicine approaches to modulate stem cells in brain repair

Trends in Biotechnology, 34(6), 437-439. *Co-corresponding authors, 2016

Akhilesh Rai, Sandra Pinto, Tiago Velho, Catarina Moita, André F. Ferreira, Urvish Trivedi, Marta Evangelista, Michela Comune, Kendra P. Rumbaugh, Pedro N. Simões, Luís Moita, Lino Ferreira

One-pot synthesis of peptide-conjugated gold nanoparticles with in vivo antimicrobial efficacy in a systemic infection model

Biomaterials, 85, 99-110, 2016

Blondel, S., Egesipe, A.-L., Jaskowiak, A.-L., Tournois, J., Le Corf, A., Georges, P., Legraverend, M., Navarro, C., Pitrez, P.R., Ferreira, L., Bollot, G., Bauvais, C., Laustriat, D., De Sandre, Giovannoli, A., Levy, N., Peschanski, M., Nissan, X.

Pluripotent stem cells-based screening identifies aminopyrimidines as new modulators of prelamin A farnesylation for the treatment of Hutchinson Gilford progeria syndrome

Cell Death and Differentiation, 7:e2105, 2016

Ferreira, R., Fonseca, M.C., Santos, T., Sargento-Freitas, J, Tjeng, R., Paiva, F., Castelo-Branco, M., Ferreira, L.S., Bernardino, L.

Retinoic acid-loaded polymeric nanoparticles enhance vascular regulation of neural stem cell survival and differentiation after ischaemia

Nanoscale, 8, 8126-8137, 2016

Aday, S., Cecchelli, R., Vanhuxeem, D., Dehouck, MP., Ferreira, L.

Stem cell-based human blood brain barrier models for drug discovery and delivery

Trends in Biotechnology, 34(5), 382-393, 2016

Cláudia Saraiva, Lino Ferreira, Liliana Bernardino

Traceable microRNA-124 loaded nanoparticles as a new promising therapeutic tool for Parkinson’s disease

Neurogenesis, 3 (1), e1256855, 2016


Annachiara De Sandre-Giovannoli, MD, PhD; Rabah Ben Yaou, MD; France Leturcq; Carlos López-Otín; Xavier Nissan, PhD; Lino Ferreira, PhD; Catherine Coirault, MD, PHD; Catherine Badens, PhD; Nathalie Bonello-Palot; Claudia Cavadas, PhD; Antoine Muchir, PhD; Camilla Pellegrini, PhD; Karim Harhouri, PhD; Chiara Lanzuolo, PhD; Feriel Azibani, PhD; Brigitte Buendia, PhD; Pia Bernasconi; Patrice Roll, Md, PhD; Isaline Herrada; Sophie Zinn-Justin, PhD; Esma Ziat; Lorenzo Maggi, MD; Saida Ortolano, MD,PhD; Marie-Christine Vantyghem, MD, PhD; Faiza Bensmaine, MD; Michael Mitchell, PhD; Raoul CM Hennekam, MD, PhD; Susana Quijano-Roy, MD, PhD; Adele D’Amico, MD; Giuseppe Boriani, MD; Elena Biagini, MD; Karim Wahbi, MD, PhD; Denis Duboc, MD, PhD; Nicola Carboni, MD; Camille Vatier, MD, PhD; Corinne Vigouroux, MD, PhD; Tiziana E Mongini, MD; Alessandra Gambineri, MD; Christophe Béroud, PharmD, PhD; Gaëlle Blandin, PhD; Sara Benedetti, MD, PhD; David Araújo-Vilar, MD; Luigi Ravagnan; Ryszard Kole, PhD; Nicolas Levy, MD, PhD; Giovanna Lattanzi, PhD; Gisèle PhD Bonne, PhD Orphanet

An overview of new translational, clinical and therapeutic perspectives in laminopathies and other nuclear envelope-related diseases

Proceedings of the 1st French-Italian meeting on laminopathies & other nuclear envelope-related diseases. Orphanet Journal of Rare Diseases, 10(Suppl 2):O1, 2015

Ivana Kostic*, Isabel Fidalgo-Carvalho*, Sezin Aday, Helena Vazão, Tiago Carvalheiro, Mário Grãos, António Duarte, Carla Cardoso, Lino Gonçalves, Lina Carvalho, Artur Paiva, Lino Ferreira

Lysophosphatidic acid enhances survival of human CD34+ cells in ischemic conditions mainly through peroxisome proliferator-activated receptor g

Scientific Reports, 5:16406, 2015

Esteves, M., Saraiva, T., Rocha,S., Baltazar,G., Ferreira,L., Bernardino, L.

Retinoic acid-loaded polymeric nanoparticles induce neuroprotection in a mouse model of Parkinson’s disease

Frontiers in Aging Neurosciences, 7, 20, 2015

Pitrez, P., Rosa, S., Almeida, C., Nissam, X., Ferreira, L.

Vascular disease modeling using induced pluripotent stem cells: focus in Hutching-Gilford progeria syndrome

Biochemical and Biophysical Research Communications, 2015


N. Lang*, M.J. Pereira*, I. Friehs, N. Vasilyev, E. N. Feins, K. Ablasser, E. O’Cearbhaill, C. Xu, A. Fabozzo, Y. Lee, R. Padera, S. Wasserman, F. Freudenthal, L.S. Ferreira, R. Langer, J.M. Karp, P. J. del Nido

A biocompatible light-activated adhesive for minimally invasive repair of cardiovascular defects

Science Translational Medicine, 6(218), 218ra6. *Authors contributed equally, 2014

Cechelli R.*, Aday S.*, Sevin E., Almeida C., Culot M., Dehouck L., Coisne C., Engelhardt B., Dehouck M.-P., Ferreira L.

A stable and reproducible human blood-brain barrier model derived from stem cells

PLoS ONE, 9 (6), e99733. doi:10.1371/journal.pone.0099733. * Authors contributed equally, 2014

Chereddy, K.K., Her, C.-H., Moia, C., Comune, M., Vanacker, J., Zhu, H., Ferreira, L.S., Vandermeulen, G., Préat, V.

Antimicrobial peptide LL37-loaded PLGA nanoparticles promote wound healing activity

J Control Release, 194, 138-147, 2014

Ricotti, L. Neves, R., Ciofani, G., Canale, C., Nitti, S., Mattoli, V., Mazzolai, B., Ferreira, L., Menciassi, A.

Boron nitride nanotube-mediated stimulation modulates F/G-actin ratio and mechanical properties of human dermal fibroblasts

Journal of Nanoparticle Research, 16(2), 1-14, 2014

Aday S.*, Paiva J.*, Sousa S., Gomes R.S.M., Pedreiro S., So P.W., Carr C.A., Cochlin L., Gomes A.C., Paiva A., Ferreira L.

Inflammatory modulation of hematopoietic stem cells by Magnetic Resonance Imaging (MRI)-detectable nanoparticles

RSC Advances, 4, 31706-31709. * Authors contributed equally, 2014

Santos, S.M., Peixoto, F., Ferreira, L., Jurado, A.S., Videira, R.A.

Interaction of fullerene nanoparticles with biomembranes: from the partition in lipid membranes to effects on mitochondrial bioenergetics

Toxicological Sciences, 138(1), 117-129, 2014


Pereira, MJ, Oyuang, B, Sarkar, D, Lang, N, Sunbdback, C, Fiehs, I, Mureli, S, Pometantseva, I, McFaden, J, Sehn, JD, Mwizerwa, O, del Nido, P, Masiakos, P, Langer, R, Ferreira, LS*, Karp, JM*

A highly tunable elastomer for local and controlled release of macromolecular therapeutics

Advanced Materials, 25(8), 1209-1215. *Authors contributed equally, 2013

Lino, MM*, Paulo, CSO*, Vale, AC, Vaz, MF, Ferreira, LS

Antifungal activity of dental resins containing amphotericin B-functionalized silica nanoparticles

Dental Materials, 29(10): e252-62. *Authors contributed equally, 2013

Ricotti, L, Fujie, T, Vazão, H, Ciofani, G, Filippeschi, C, Corradini, I, Matteoli, M, Mattoli, V, Ferreira, L, Menciassi, A

Boron nitride nanotube-mediated stimulation of cell co-culture on microengineered hydrogels

PLoS One, 8(8), e71707, 2013

Pereira, MJN, Sundback, CA, Lang, N, Cho, WK, Pomerantseva, I, Ouyang, B, Tao, SL, McHugh, K, Mwizerwa, O, Vemula, PK, Mochel, MC, Carter, DJ, Borenstein, JT, Langer, R, Ferreira, LS*, Karp, JM*, Masiakos, PT*

Combined surface micropatterning and reactive chemistry maximizes tissue adhesion with minimal inflammation

Advanced Health Materials, doi: 10.1002/adhm.201300264. *Corresponding authors, 2013

Paulo, CSO*, Lino, MM*, Matos, AA, Ferreira, LS

Nanoparticles conjugated with amphotericin B are differentially taken up by human cells and after internalization increase the expression of heat shock protein

Biomaterials, 34(21), 5281-5293. *Authors contributed equally, 2013

Gomes, RSM, Neves, R, Cochlin, L, Lima, AF, Carvalho, R, Korpisalo, P, Dragneva, G, Turunen, M, Liimatainen, T, Clarke, K, Yla-Herttuala, S, Carr, CA, Ferreira, L

Nanoparticles for simultaneous cell tracking and pro-survival/angiogenic miRNA delivery in an ischemic animal model

ACS Nano, 7(4), 3362-3372, 2013


Ferreira, R, Santos, T, Gonçalves, J, Baltazar, G, Ferreira, L, Agasse, F, Malva, JO, Bernardino, L

Histamine modulates microglial inflammatory response

Journal of Neuroinflammation, 9:90, doi: 10.1186/1742-2094-9-90, 2012

Bernardino, L, Eiriz, MF, Santos, T, Xapelli, S, Grade, S, Rosa, A, Cortes, L, Ferreira, R, Bragança, J, Agasse, F, Ferreira, L, Malva, JO

Histamine, a proneurogenic factor on the subventricular zone: a new approach for brain repair

Stem Cells, 30, 773-784, 2012

Santos, T, Maia, J, Agasse, F, Xapelli, S, Ferreira, L, Bernardino, L

Nanomedicine boosts neurogenesis: new strategies for brain repair

Integrative Biology, 4, 973-981, 2012

Botequim, D, Maia, J, Lino, MMF, Lopes, LMF, Simoes, PN, Ilharco, LM, Ferreira, L

Nanoparticles and surfaces presenting antifungal, antibacterial and antiviral properties

Langmuir, 28(2), 7646-7656, 2012

Santos, T, Ferreira, R, Maia, J, Agasse, F, Xapelli, S, Cortes, L, Bragança, J, Malva, JO, Ferreira, L*, Bernardino, L*

Polymeric nanoparticles to control the differentiation of neural stem cells in the subventricular zone of the brain

ACS Nano, 6(12), 10463-10474. *Authors contributed equally, 2012

Ricotti, L, Polini, A, Genchi, G, Ciofani, G, Iandolo, D, Vazão, H, Mattoli, V, Ferreira, L, Menciassi, A, Pisignano, D

Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibous PHB scaffolds

Biomedical Devices, 7(3), 035010, 2012

Maia*, J, Vazão*, H, Pedroso, DCS, Jesus, C, Brito, RMM, Gil, MH, Ferreira, L

VEGF functionalized dextran has longer intracellular bioactivity than VEGF in endothelial cells

Biomacromolecules, 13, 2906-2916. *Authors contributed equally, 2012


Kraehenbuehl, TP, Langer, R*, Ferreira, LS*

Biomaterials for the self-renewal and differentiation of human pluripotent stem cells

Nature Methods, 8(9), 731-736. *Corresponding authors, 2011

Maia, J, Santos, T, Aday, S, Agasse, F, Cortes, L, Malva, JO, Bernardino, L, Ferreira, L

Controlling the neuronal differentiation of stem cells by the intracellular delivery of retinoic acid-loaded nanoparticles

ACS Nano, 5(1), 97-106. Authors contributed equally. L F and LB are corresponding authors, 2011

Kraehenbuehl, T.P., Ferreira, L.S.*, Hayward, A.M., Nahrendorf, M., Vlies, A.J., Vasile, E., Weissleder, R., Langer, R.*, Hubbell, J.A.*

Human embryonic stem cell-derived microvascular grafts for cardiac tissue preservation after myocardial infarction

Biomaterials, 32(4), 1102-1109. *Corresponding authors, 2011

Pedroso, DCS, Tellechea, A, Moura, L, Fidalgo-Carvalho, I, Duarte, J, Carvalho, E, Ferreira, L

Improved survival, vascular differentiation and wound healing potential of umbilical cord stem cells co-cultured with endothelial cells

PLoS One, 6(1):e16114, 2011

Paulo, CSO, Pires das Neves, R, Ferreira, LS

Nanoparticles for intracellular-targeted drug delivery

Nanotechnology, 22, 494002, 2011

Nunes-Pereira, MJ, Fidalgo-Carvalho, I, Karp, JM*, Ferreira, LS*

Sensing the cardiac environment: exploiting cues for regeneration

Journal of Cardiovascular Translational Research, 4, 616-630. *Corresponding authors, 2011

Vazão, H, Pires das Neves, R, Grâos, M, Ferreira, L

Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells

PLoS One, 6(3): e17771, 2011


Paulo, C, Vidal, M, Ferreira, LS

Antifungal nanoparticles and surfaces

Biomacromolecules, 11, 2810-2817, 2010

Levenberg, S*, Ferreira LS*, Chen-Konak, L, Kraehenbuehl, TP, Langer, R

Isolation, differentiation, and characterization of vascular cells derived from human embryonic stem cells

Nature Protocols, 5, 1115-1126. *Authors contributed equally, 2010

Ferreira, L*, Pedroso, DCS, Vazão, H, Gomes, RSM

Stem cell-based therapies for heart regeneration: what did the bench teach us?

Cardiovascular & Haematological Disorders-Drug Targets, 10, 173-185. *Corresponding author, 2010


Kraehenbuehl TP, Ferreira LS, Zammaretti P, Hubbell JA, Langer R

Cell-responsive hydrogel for encapsulation of vascular cells

Biomaterials, 30(26), 4318-4324, 2009

Ferreira LS

Nanoparticles as tools to study and control stem cells

Journal of Cellular Biochemistry, 108(4), 746-752, 2009

Ferreira LS*, Zumbuehl A*

Non-leaching surfaces capable of killing microorganisms on contact

Journal of Materials Chemistry, 19, 7796-7806. *Corresponding authors., 2009


Mahdavi A*, Ferreira L*, Sundback C, Nichol JW, Chan EP, Carter JD,Bettinger C, Patanavanich S, Chignozha L, Ben-Joseph E, Galakatos A, Pryor H, Pomerantseva I, Masiakos P, Faquin W, Zumbuehl A, Hong S, Borenstein J, Vacanti J, Langer R, Karp JM

A biodegradable and biocompatible Gecko-inspired tissue adhesive

Proceedings of the National Academy of Sciences, 105(7), 2307-2312. *Authors contributed equally, 2008

Ferreira L, Squier T, Park H, Choe H, Kohane DS, Langer R

Human embryoid bodies containing nano- and micro-particulate delivery vehicles

Advanced Materials, 20, 2285-2291, 2008

Fuller, J., Zugates, G., Ferreira, L., Ow, H., Nguyen, N., Wiesner, U., Langer, R.

Intercellular delivery of core-shell fluorescent silica nanoparticles

Biomaterials, 29, 1526-1532, 2008

Ferreira L*, Karp JM, Nobre L, Langer R*

New opportunities: the use of nanotechnologies to manipulate and track stem cells

Cell Stem Cell, 3(2), 136-146. *Corresponding authors, 2008


Zumbuehl, A.*, Ferreira, L.S.*, Kuhn, D., Astashkina, A., Long, L., Yeo, Y., Iaconis, T., Ghannoum, M., Fink, G.R., Langer, R., Kohane, D.S.

Antifungal hydrogels

Proceedings of the National Academy of Sciences, 104, 12994-12998. *Authors contributed equally, 2007

Ferreira, L., Gerecht, S, Fuller, J, Shieh, HF, Vunjak-Novakovic, G, Langer, R

Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells

Biomaterials, 28(17), 2706-2717, 2007

Nijst, C., Joost, J., Karp, J.M., Ferreira, L., Zumbuehl, A., Bettinger, C., Langer, R.

Development of Multifunctional Photocurable Degradable Elastomers

Biomacromolecules, 8, 3067-3073, 2007

Gerecht-Nir, S., Burdick, J.A., Ferreira, L.S., Langer, R., Vunjak-Novakovic, G.

Propagation of undifferentiated human embryonic stem cells in 3-dimentional hyaluronic acid networks

Proceedings of the National Academy of Sciences, 104(27), 11298-11303, 2007

Ferreira, L, Gerecht-Nir, S., Shieh, H.F., Watson, N., Rupnick, M, Dallabrida, S, M, Vunjak-Novakovic, G., Robert Langer, R.

Vascular progenitor cells isolated from human embryonic stem cells give rise to endothelial and smooth muscle-like cells and form vascular networks in vivo

Circulation Research, 101, 286-294, 2007


Ferreira, L.*, Khademhosseini, A.*, Blumling, J, Yeh, J., Karp, J.M., Fukuda, J., Langer, R.

Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates

Biomaterials, 27, 5968-5977. *Authors contributed equally, 2006

Jeffrey M. Karp, Lino S. Ferreira, Ali Khademhosseini, Albert H. Kwon, Judy Y. Yeh, Robert Langer

Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro

Stem cells, 24(4), 835-843, 2006

Ferreira, L., Figueiredo, M.M., Gil, M.H., Ramos, M.A.

Structural analysis of dextran-based hydrogels obtained chemoenzymatically

Journal of Biomedical Materials Research: part B (Applied Biomaterials), 77B:55-64, 2006


Ferreira, L., Gil, M.H., Cabrita, A.M.S., Dordick, J.S.

Biocatalytic synthesis of highly ordered biocompatible and degradable dextran-based hydrogels

Biomaterials, 26, 4707-4716, 2005

Ferreira, L., Evangelista M.B., Martins M.C.L., Esteves, J.L., Barbosa, M.A.

Improving the adhesion of poly(ethylene terephthalate) fibers to poly(hydroxyethyl methacrylate) hydrogels by ozone treatment: surface characterization and pull-out tests

Polymer, 46, 9840-9850, 2005

Maia, J., Ferreira, L., Carvalho, R., Ramos M.A., Gil, M.H.

Synthesis and characterization of new injectable and degradable dextran-based hydrogels

Polymer, 46, 9604-9614, 2005


Ferreira, L., Rafael, A., Lamghari, M., Barbosa, M.A., Gil, M.H., Cabrita, A.M.S., Dordick, J.S.

Biocompatibility of chemoenzymatically derived dextran hydrogels

Journal of Biomedical Materials Research, 68A, 584-596, 2004


Ferreira, L., Ramos, M.A., Dordick, J.S., Gil, M.H.

Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (subtilisin Carlsberg)

Journal of Molecular Catalysis B: Enzymatic, 21, 189-199, 2003


Ferreira, L., Kim, D.-Y., Carvalho, R., Geraldes, C.F.G.C., Gil, M.H., Dordick, J.S.

Biocatalytic polytransesterification of inulin with divinyladipate

Chemistry of Materials, 14(10), 4009-4011, 2002

Ferreira, L., Gil M.H., Dordick, J.S.

Enzymatic synthesis of dextran-containing hydrogels

Biomaterials, 23, 3957-3967, 2002

Ferreira, L., Carvalho, R., Gil, M.H., Dordick, J.S.

Enzymatic synthesis of inulin-containing hydrogels

Biomacromolecules, 3, 333-341, 2002

Ferreira, L., Ramos, M.A., Gil, M.H., Dordick, J.S.

Exquisite regioselectivity and transesterification activity enhancement of an immobilized Bacillus subtilis protease

Biotechnology Progress, 18(5), 986-993, 2002


Ferreira, L., Vidal, M.M., Gil, M.H.

Design of a drug-delivery system based on polyacrylamide hydrogels. Evaluation of structural properties

Chem. Educator, 6, 100-103, 2001


Ferreira, L., Vidal, M.M., Gil, M.H.

Evaluation of poly (2-hydroxyethyl methacrylate) gels as drug delivery systems at different pH values

International Journal of Pharmaceutics, 194(2), 169-180, 2000

Ferreira, L., Vidal, M.M., Geraldes, C.F.G.C., Gil, M.H.

Preparation and characterisation of gels based on sucrose modified with glycidyl methacrylate

Carbohydrate Polymers, 41(1), 15-24, 2000

Book Chapters


Estronca, L., Ferreira, L.

Stem cells for the regeneration of chronic wounds: a translational perspective

Editor: Joaquim Cabral, Elsevier, 2015


Rai, A., Pinto, S., Paulo, C. Comune, M., Ferreira, L.

Biocompatibility issues of organic and inorganic nanomaterials

Editor: Huijun Zhu, Bentham e-books, 2013


Fernandes, H., Beijersbergen, R., Ferreira, L., Dechering, K., Moghe, P., Maniura-Weber, K.

Bioassay development

In: “Materiomics: high-throughput screening of biomaterial properties”. Editors: Jan de Boer and Clemens A. Van Blitterswijk. Cambridge University Press, 2012

Maia, J., Evangelista, M. B., Gil, M. H., Ferreira, L.

Dextran-based materials for biomedical applications

Editor: Maria Helena Gil, Research Signpost, 2012


Valente, AXCN*, Sousa, JAB, Outeiro, TF, Ferreira, L

A stem-cell ageing hypothesis on the origin of Parkinson´s disease

In “Science and Engineering in high-throughput biology including a theory on Parkinson´s disease. Editor: André X.C.N. Valente. Lulu (*Corresponding author), 2011

Vazão, H., Grãos, M., Ferreira, L.

Vascular differentiation of human pluripotent stem cells

Editor: Hossein Baharvand, Springer, 2011


Cho, WK, Pereira, MJMN, Lang, N, Lee, K, Mureli, S, Ferreira, L, Langer, RS, Karp, JM

Gecko-inspired tape-based adhesives

In: “Novel Biomaterials for wound closure”. Editor: Pan Stanford Publishing, 2010

Kraehenbuehl T. P., Aday S., Ferreira L. S.

Scaffolding for three-dimensional embryonic vasculogenesis

In: “Biophysical regulation of vascular differentiation and assembly”, Editor: Sharon Gerecht. Springer, 2010


Sarkar D., Zhao W., Schaefer S., Ankrum J. A., Teo G. S., Pereira M. N., Ferreira L., Karp J. M.

Overview of tissue engineering concepts and applications

In: Biomaterials Science: an introduction to materials in medicine”, 3rd edition, Editors: Ratner, BD, Hoffman, AS, Schoen, FJ, Lemons, JE. Academic Press, 2009


Spelke, D.P., Ortmann, D., Khademhosseini, A., Ferreira, L., Karp, J.M.

Method for EB formation- the microwell approach

In: “Embryonic stem cell therapy for osteodegenerative diseases”, Series Methods in Molecular Biology, Editor: Nieden, Nicole. Humana Press, 2008

Ferreira, L.*, Gerecht, S.*, Langer, R.

Vascular differentiation of human embryonic stem cells in bioactive hydrogel scaffolds

In : “Human embryonic stem cells- methods and protocols”. Editors: Turksen, Walker. Humana Press (*Authors contributed equally), 2008


Ferreira*, L., Khademhosseini*, A., Karp*, J.M., Gerecht-Nir*, S., Vunjak-Novakovic, G., Langer, R.

Embryonic stem cells as a cell source for tissue engineering

In: “Principles of Tissue Engineering- 3rd edition”. Editors: Lanza, R., Langer, R., Vacanti, J.P. Elsevier/Academic Press (*Authors contributed equally), 2006

Karp, J. M., Mahdavi, A., Ferreira, L.S., Khademhosseini, A., Langer, R.

In vitro differentiation of human embryonic stem cells along osteogenic pathways

In: “Human Embryonic Stem Cells: A Practical Handbook”, edited by: Sullivan S, Cowan C, Eggan K. John Wiley & Sons, Ltd., 2006

Luo, Y., Ferreira, L., Langer, R.

Three-dimensional scaffolds

In: “Principles of Tissue Engineering- 3rd edition”. Editors: Lanza, R., Langer, R., Vacanti, J.P. Elsevier/Academic Press, 2006


Gil M.H., Duarte, M.G., Ferreira, L., Geraldes, C.F.G.

Polymeric Biomaterials

In: Polymer Chemistry, Eds: Melo, J.S.S., Moreno, M.J., Burrows, H.D., Gil, M.H. Coimbra University Press, Coimbra, p. 641-664, 2004



Lino, M., Ferreira, L.

A composition for controlled release of proteins

PAT20151000082123, 2015

Comune, M., Ferreira, L.

A composition for use in the treatment of wound healing or in the treatment of ischemic aor vascular diseases

PAT20151000082124, 2015

Pitrez, P., Vazão, H., Ferreira, L.

Treatment of Hutchinson-Gilford Progeria Syndrome and diseases related to vascular ageing

PAT20151000003657, 2015


Cecchelli, R., Aday, S., Sevin, E., Ferreira, L.

A human blood-brain barrier model derived from stem cells

PAT20131000023338, 2013

Boto, C., Neves, R., Ferreira, L.

Light-activatable polymeric nanoparticles

PAT20131000066281, 2013


Lino Ferreira, Akhilesh Rai

Antimicrobial coating compositions

PAT20121000070064, 2012

Lino Ferreira, David Botequim, João Maia

Antimicrobial complexes

EP12398001, 2012

Lino Ferreira, Helena Vazão

Assessment of embryonic vascular toxicity by pluripotent stem cell-derived arterial endothelial cells on a microfluidic system

PAT20121000095173, 2012

Lino Ferreira, Isabel Carvalho

Composition and method of stem cells for preservation of cardiac tissue

PAT20121000032891, 2012


Lino Ferreira, Dora Pedroso

Composition and process to improve the therapeutic effect of stem cells

PCT/IB2011/055342, 2011

Lino Ferreira, Renata Gomes, Ricardo Neves

Nanoparticles and uses thereof

EPA 11398008.0, 2011


Lino Ferreira, Cristiana Paulo

Antimicrobial nanoparticle conjugate

Pending Patent, 2010

Lino Ferreira, Dora Pedroso

Method for improving the survival, vascular differentiation and wound healing potential of stem cells by co-culturing with endothelial cells in a biomimetic gel

Pending Patent, 2010

Maia, J, Santos, T, Malva, JO, Bernardino, L*, Ferreira, L*

Nanoparticles to control stem cells

Pending Patent, 2010


Lino Ferreira, Alborz Mahdavi, Cathryn Sundback, David J. D. Carter, Chris Bettinger, Andreas Zumbuehl, Jeff Borenstein, Joseph Vacanti, Robert Langer, Jeffrey M. Karp

A biodegradable and biocompatible Gecko-inspired tissue adhesive

WO2009067482, 2009

Ferreira, L., Kohane, D., Langer, R.

Human embryoid bodies containing nano- and micro-particulate delivery vehicles

WO2009100128, 2009


Zumbuehl, A., Ferreira, L.S., Kohane, D.S., Fink, G.R., Langer, R.S.

Coating of devices with gel, foam, film, particle or compn, comprising a polymer solvent and effector compounds, and their use for preventing infection and treatment of diseases

WO2008005297, 2008


Karp, J.M., Ferreira, L.S., Khademhosseini, A., Langer, R.S.

Amplification of specific cell populations from human embryonic stem cells

WO2007022348, 2007

Nijst, C., Karp, J.M., Joost, J., Ferreira, L., Zumbuehl, A., Bettinger, C., Langer, R.

Development of Multifunctional Photocurable Degradable Elastomers

WO2007082304 and WO2007082305, 2007

Ferreira, L.S., Gerecht-Nir, S., Vunjak-Novakovic, G., Langer, R.S.

Formation of vascular networks using human embryonic stem cells

WO2007140340, 2007

Christopher Loose, William Shannan O´Shaughnessy, Lino Ferreira, Andreas Zumbuehl, Robert Langer, Gregory Stephanopolous

Medical devices and coatings with non-leaching bioactive peptides

WO2007095393, 2007